RNA

Part:BBa_K3645017:Design

Designed by: Huang nan   Group: iGEM20_Peking   (2020-10-27)


sgRNA scaffold with 2xMS2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 178
    Illegal PstI site found at 248
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 178
    Illegal PstI site found at 248
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 178
    Illegal PstI site found at 248
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 178
    Illegal PstI site found at 248
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 135
    Illegal BsaI.rc site found at 37


Design Notes

This part already contains a promoter and terminator domain, only template sequence replacement is needed


Source

Madej T, Lanczycki CJ, Zhang D, Thiessen PA, Geer RC, Marchler-Bauer A, Bryant SH. " MMDB and VAST+: tracking structural similarities between macromolecular complexes. Nucleic Acids Res. 2014 Jan; 42(Database issue):D297-303

References